譜析（上海）生物科技有限公司優(yōu)勢供應：T4 DNA Ligase
2019/10/12 14:57:07
Inspiralis提供T4 DNA連接酶已有多年。我們可以少量供應，也可以大量供應，或者更集中供應(例如，用于dna標記化學圖書館建設)。T4 DNA連接酶催化磷酸二酯鍵在末端5磷酸和3個雙鏈DNA或RNA羥基之間形成。它可以連接DNA或寡核苷酸的粘性末端，以及RNA和RNA-DNA的雜交體，在雙鏈DNA上尤其有效。它也可以連接鈍端DNA，但不能連接單鏈核酸。它需要ATP作為輔助因子。T4 DNA連接酶以2000 u /ul或13000 u /ul的速度供應，并提供10X緩沖。1U是一個內聚的末端連接單元(CELU)。650個CELUs大約是一個Weiss單元，所以2000個CELUs/ul相當于~3個Weiss單元/ul (TDL1, TDL2)或20個Weiss單元/ul (TDL3C)
Inspiralis has been supplying T4 DNA ligase for many years. We can supply it in the smaller quantities below or in larger or more concentrated quantities (for example for DNA-tagged chemical library construction).
T4 DNA ligase catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. It can ligate cohesive ends of DNA or oligonucleotides, as well as RNA and RNA-DNA hybrids and is particularly efficient at sealing nicks in duplex DNA. It can also ligate blunt-ended DNA but cannot ligate single-stranded nucleic acids. It requires ATP as a cofactor.
T4 DNA ligase is supplied at either 2,000U/ul or 13,000U/ul along with 10X buffer. 1U is a cohesive end joining unit (CELU). 650 CELUs is approximately 1 Weiss unit so 2000 CELUs/ul is equivalent to ~3 Weiss units/ul (TDL1, TDL2) or 20 Weiss units/ul (TDL3C)
優(yōu)勢供應產品：
譜析（上海）生物科技有限公司是一家致力于為實驗室分析領域
更多產品請咨詢上海熹垣生物。
Inspiralis has been supplying T4 DNA ligase for many years. We can supply it in the smaller quantities below or in larger or more concentrated quantities (for example for DNA-tagged chemical library construction).
T4 DNA ligase catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. It can ligate cohesive ends of DNA or oligonucleotides, as well as RNA and RNA-DNA hybrids and is particularly efficient at sealing nicks in duplex DNA. It can also ligate blunt-ended DNA but cannot ligate single-stranded nucleic acids. It requires ATP as a cofactor.
T4 DNA ligase is supplied at either 2,000U/ul or 13,000U/ul along with 10X buffer. 1U is a cohesive end joining unit (CELU). 650 CELUs is approximately 1 Weiss unit so 2000 CELUs/ul is equivalent to ~3 Weiss units/ul (TDL1, TDL2) or 20 Weiss units/ul (TDL3C)
優(yōu)勢供應產品：
| CatNo. | Product |
| TDL1 | Kit 1 80,000U T4 DNA ligase (2000U/ul), 10X buffer |
| TDL2 | Kit 2 400,000U T4 DNA ligase (2000U/ul), 10X buffer |
| TDL3C | Kit3 400,000U T4 DNA ligase (13,000U/ul), 10X buffer |
